Glucose oxidase (GOD) is a typical oxidoreductase found in honey and moulds such as Penicillium notatum. It can highly specifically catalyze the β-D-glucose reaction to generate gluconic acid, which has the function of converting glucose and removing oxygen. Since GOD can quantitatively generate hydrogen peroxide, it is widely used in the field of biochemistry and clinical examination as a quantitative reagent for D-glucose.
Glucose oxidase (GOD) is a typical oxidoreductase found in honey and molds such as Penicillium notatum. It can highly specifically catalyze the β-D-glucose reaction to generate gluconic acid, which has the function of converting glucose and removing oxygen. Since GOD can quantitatively generate hydrogen peroxide, it is widely used in the field of biochemistry and clinical examination as a quantitative reagent for D-glucose.
GOD can be used in biochemical research, and is clinically often used as a diagnostic enzyme, for glucose analysis, preparation of urine glucose and blood glucose test paper, and as a stabilizer for vitamin C and B12 preparations in the pharmaceutical industry. The refined enzyme can be used for clinical diagnosis, quickly and accurately determine the glucose content in body fluids, and provide reliable data for doctors to accurately determine the patient's condition.
The commonly used glucose oxidase method for measuring blood sugar includes the following two products:
Clinically, blood glucose determination kits (glucose oxidase method) are mainly used to quantitatively determine the content of glucose in human serum. It is small in size, easy to carry, does not require special training, and can quickly detect blood glucose results at any time. The principles of the glucose oxidase method to determine glucose in serum are as follows:
Due to the poor specificity of the Trinder reaction, some reducing substances such as uric acid, vitamin C, bilirubin and glutathione can compete with chromogenic substances for hydrogen peroxide. Thus, the hydrogen peroxide produced during the reaction is consumed, and competitive inhibition is produced, which makes the measurement result low.
We often see that blood glucose test strips need to be used with a glucose oxidase blood glucose meter, which has high specificity for glucose and relatively accurate results. However, because the reaction process requires the participation of oxygen, the measurement result is easily affected by the blood oxygen content and the result is biased. The test paper is also easy to react with oxygen in the air. Generally, it should be used up within 3 to 4 months after opening, and the shelf life is relatively short.
The Naming and Characteristics of Restriction Endonucleases
Restriction enzymes, also known as restriction endonucleases, are enzymes that cut double-stranded DNA. Its cutting method is to cut the bond between the carbohydrate molecule and the phosphoric acid, and then create a nick on each of the two DNA strands without damaging the nucleotides and bases.
Introduction to Classification and Application of Cellulase
The optimum pH of cellulase is generally 4.5 to 6.5, which acts on cellulose and products derived from cellulose. Microbial cellulase is of great significance in converting insoluble cellulose into glucose and destroying cell walls in fruit and vegetable juice to improve the yield of juice.
Which Enzyme Preparations Are Often Used in Fruit and Vegetable Processing?
The so-called fruit and vegetable processing means to maximize the nutrient content of the fruit and vegetable through various processing methods, improve the edible value, and make the color, aroma and taste of the processed product more perfect.
