Oligonucleotide Synthesis in Molecular Biology
In a strict sense, an oligonucleotide is a short piece of DNA or RNA which is less than 50 nucleotides in length. But in a broad sense, it also refers to all chemically synthesized nucleic acids, regardless of the length.
In a strict sense, an oligonucleotide is a short piece of DNA or RNA which is less than 50 nucleotides in length. But in a broad sense, it also refers to all chemically synthesized nucleic acids, regardless of the length. Oligonucleotides have a wide range of applications in genetic testing, research, and forensics.
The synthesis of oligonucleotide has long been an important technique experiment in laboratories. Oligonucleotide synthesis means the process of using chemical approach to synthesize relatively short fragments of nucleic acids with defined chemical structure. The technique has become extremely important in current laboratory practice in that it provides a rapid and inexpensive approach to produce customized oligonucleotides of desired sequence.
Synthetic oligonucleotides are essential for the development of molecular biology. Nearly every current technique used in molecular biology requires chemically synthesized DNAs or RNAs, such as PCR, Real-Time PCR, DNA sequencing, site directed mutagenesis, single-nucleotide polymorphism assays, microarrays. Oligonucleotides can not be bulk stored for use as each research requires specific oligonucleotide. They are custom made according to specific application required by the research.
Millions of oligonucleotides are synthesized every year for use in laboratories around the world. Small quantities of oligonucleotides are enough for most applications. Oligonucleotide synthesis can provide ample quantities for most biochemical and biological experiments. For more large scale production of chemical oligonucleotides, solid-phase methods are required. The solid-phase methods have been developed to provide much larger amounts of DNA for use in biophysical studies and drug molecules.
The solid-phase synthesis has been widely used in peptide synthesis, oligonucleotide synthesis, oligosaccharide synthesis and combinatorial chemistry. This synthesis method is carried out on a solid support held between filters, in columns that enable all reagents and solvents to pass through freely, and it has a number of advantages. Such as the process can be automated on computer-controlled solid-phase synthesizers.
In recent years, advances in oligonucleotide synthesis chemistries and quality control technologies have led to substantial increases in both quality and yield. Many companies, such as Creative Biogene, can provide a full spectrum of high quality specialty oligonucleotide synthesis service that range from oligo synthesis for both research and cGMP grade, oligonucleotide modifications to high throughput DNA, RNA oligo synthesis.The custom DNA oligos are optimized for various synthesis scales, modifications and applications to ensure consistently high quality and fast turnaround time.
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