Resveratrol increases chemotherapeutic sensitivity of CNE2 cells in nasopharyngeal carcinoma

Decreasing of memory-ray sensitivity in solid tumors and hypoxic cells of chemotherapy drug resistance are the root for the failures of tumor radiotherapy and chemotherapy, tumor recurrence and regrowth.Depth study of nasopharyngeal carcinoma hypoxic cell multidrug resistance mechanism and to find an effective sensitizer or sensitizing method has become the hot topic in the current clinical treatment. Resveratrol is a multi-phenolic compound widely found in grapes, peanuts and a variety of traditional Chinese medicines. It can lower blood pressure, anti-inflammatory, antioxidant, inhibit tumor cell proliferation, metastasis and promote apoptosis and other biological functions. It is a hot research topic in the field of integrative medicine in recent years. The study finds that resveratrol will increase chemotherapy sensitivity on multidrug resistance KBv200 cells.

In recent years, a large number of clinical studies have shown that radiation therapy combined with chemotherapy can achieve a higher cure rate, but due to the rapid growth of malignant cells, tumor tissue perfusion uneven, resulting in local tissue within the tumor, acute and chronic hypoxia, and a series of biological changes caused by the tumor cells. Hypoxic cells of solid tumors that exist within the tumor put the failure of chemotherapy, tumor recurrence and re-growth of roots. Relatively specific hypoxic microenvironment, to find a chemosensitizer of hypoxic cells to become concerned about the hot spots. The pre-experimental study found that hypoxia by inducing the expression of the multidrug resistance-associated gene, reducing the sensitivity of tumor cells to chemotherapeutic drugs. The study of resveratrol sensitizing effect of chemotherapy for nasopharyngeal carcinoma cells under hypoxic environment.

The lack of oxygen caused by apoptosis of tumor cells decreased ability to make certain chemotherapy drugs lose efficacy and tumor resistance. Looking for low toxicity and few adverse reactions chemosensitizer to enhance the therapeutic effect is important. Resveratrol, that is, non-flavonoid polyphenol compounds, 3,5,4 '-trihydroxy-stilbene, is a phytoalexin of plant resistance to outside stimuli such as ultraviolet light, fungus, virus infection or mechanical damage. Non-cytotoxic doses of resveratrol can significantly improve the cytotoxic effects of paclitaxel, cisplatin and fluorouracil on CNE2, the sensitizing effect of a concentration dependent manner, suggesting that resveratrol has the role of chemotherapy sensitivity. Resveratrol How to improve of hypoxia nasopharyngeal carcinoma cell CNE2 on chemosensitivity is unclear. Most chemotherapeutic drugs induce tumor cell apoptosis and play a role in cancer. Non-cytotoxic dose of resveratrol on paclitaxel-induced apoptosis of CNE2 cells significantly enhanced the role of the detailed mechanism needs further study.

Drug resistance and the formation of more complex mechanism known P gp and MRP resistance. P gp was an energy-dependent membrane transport proteins, can intake intracellular drug transport out of the cell, reducing the effective intracellular drug concentration. Therefore, the inherent resistance of P-gp overexpression in tumor cells. MRP1 is a non-P-gp membrane transport proteins, their mechanisms of resistance is also out through the energy-dependent drug pump, involved in drugs into the re-distribution of organelles or directly affect the drug inside the cell. Of HIF 1± is the most important nuclear transcription factor in the state of hypoxia, the regulation of the transcriptional level of target gene expression, which leads to a variety of cellular response to hypoxia.

Pharmaceutical raw materials resveratrol can significantly reduce the expression of HIF 1± and multi-drug resistance gene of mdr1, MRP1 with a concentration dependent manner. The resveratrol can lower the multidrug resistance gene in the hypoxic environment thereby enhancing the sensitivity of tumor cells. Whether it is though nuclear transcription factor of HIF 1± to regulate the multidrug resistance gene and its protein expression or has effect on HIF 1± and multidrug resistance-associated genes of mdr1 at the same time, it needs further study. Resveratrol can increase CNE2 cell sensitivity to paclitaxel. It suggests that resveratrol may be used as a chemosensitizer to increase or achieve effective chemotherapy for nasopharyngeal carcinoma.Source:http://www.cospcn.com